Diet-induced vitamin D deficiency reduces skeletal muscle mitochondrial respiration

Diet-induced vitamin D deficiency reduces skeletal muscle mitochondrial respiration.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1530/JOE-20-0233
Journal or Publication Title: Journal of Endocrinology
Volume: 249
Number: 2
Page Range: pp. 113-124
Date: 2021
Divisions: UTS Centre for Inflammation
Depositing User: General Admin
Identification Number: 10.1530/JOE-20-0233
ISSN: 0022-0795
Date Deposited: 10 Jun 2021 05:30
Abstract:

Vitamin D deficiency is associated with symptoms of skeletal muscle myopathy including muscle weakness and fatigue. Recently, vitamin D-related metabolites have been linked to the maintenance of mitochondrial function within skeletal muscle. However, current evidence is limited to in vitro models and the effects of diet-induced vitamin D deficiency upon skeletal muscle mitochondrial function in vivo have received little attention. In order to examine the role of vitamin D in the maintenance of mitochondrial function in vivo, we utilised an established model of diet-induced vitamin D deficiency in C57BL/6J mice. Mice were either fed a control diet (2200 IU/kg i.e. vitamin D replete) or a vitamin D-deplete (0 IU/kg) diet for periods of 1, 2 and 3 months. Gastrocnemius muscle mitochondrial function and ADP sensitivity were assessed via high-resolution respirometry and mitochondrial protein content via immunoblotting. As a result of 3 months of diet-induced vitamin D deficiency, respiration supported via complex I + II (CI + IIP) and the electron transport chain (ETC) were 35 and 37% lower when compared to vitamin D-replete mice (P < 0.05). Despite functional alterations, citrate synthase activity, AMPK phosphorylation, mitofilin, OPA1 and ETC subunit protein content remained unchanged in response to dietary intervention (P > 0.05). In conclusion, we report that 3 months of diet-induced vitamin D deficiency reduced skeletal muscle mitochondrial respiration in C57BL/6J mice. Our data, when combined with previous in vitro observations, suggest that vitamin D-mediated regulation of mitochondrial function may underlie the exacerbated muscle fatigue and performance deficits observed during vitamin D deficiency.

Creators:
Creators
Email
Ashcroft, Stephen P
UNSPECIFIED
Fletcher, Gareth
UNSPECIFIED
Philp, Ashleigh M
UNSPECIFIED
Jenkinson, Carl
UNSPECIFIED
Das, Shatarupa
UNSPECIFIED
Hansbro, Philip M
UNSPECIFIED
Atherton, Philip J
UNSPECIFIED
Philp, Andrew
UNSPECIFIED
Last Modified: 10 Jun 2021 05:30
URI: https://eprints.centenary.org.au/id/eprint/977

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