A 3D-printed microfluidic platform for simulating the effects of CPAP on the nasal epithelium

A 3D-printed microfluidic platform for simulating the effects of CPAP on the nasal epithelium.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1088/1758-5090%2Fabe4c1
Journal or Publication Title: Biofabrication
Volume: 13
Number: 3
Page Range: 035028
Date: 2021
Divisions: UTS Centre for Inflammation
Depositing User: General Admin
Identification Number: 10.1088/1758-5090/abe4c1
ISSN: 1758-5082
Date Deposited: 10 Jun 2021 05:20

Obstructive sleep apnoea (OSA) is a chronic disorder that involves a decrease or complete cessation of airflow during sleep. It occurs when the muscles supporting the soft tissues in the throat relax during sleep, causing narrowing or closure of the upper airway. Sleep apnoea is a serious medical condition with an increased risk of cardiovascular complications and impaired quality of life. Continuous positive airway pressure (CPAP) is the most effective treatment for moderate to severe cases of OSA and is effective in mild sleep apnoea. However, CPAP therapy is associated with the development of several nasal side effects and is inconvenient for the user, leading to low compliance rates. The effects of CPAP treatment on the upper respiratory system, as well as the pathogenesis of side effects, are incompletely understood and not adequately researched. To better understand the effects of CPAP treatment on the upper respiratory system, we developed an in vitro 3D-printed microfluidic platform. A nasal epithelial cell line, RPMI 2650, was then exposed to certain conditions to mimic the in-vivo environment. To create these conditions, the microfluidic device was utilized to expose nasal epithelial cells grown and differentiated at the air-liquid interface. The airflow was similar to what is experienced with CPAP, with pressure ranging between 0-20 cm of H20. Cells exposed to pressure showed decreased barrier integrity, change in cellular shape, and increased cell death (lactate dehydrogenase release into media) compared to unstressed cells. Stressed cells also showed increased secretions of inflammatory markers IL-6 and IL-8 and had increased production of ATP. Our results suggest that stress induced by airflow leads to structural, metabolic, and inflammatory changes in the nasal epithelium, which may be responsible for developing nasal side-effects following CPAP treatment.

© 2021 IOP Publishing Ltd.

Shrestha, Jesus
Ryan, Sean Thomas
Mills, Oliver
Zhand, Sareh
Razavi Bazaz, Sajad
Hansbro, Philip Michael
Ghadiri, Maliheh
Ebrahimi Warkiani, Majid
Last Modified: 10 Jun 2021 05:20
URI: https://eprints.centenary.org.au/id/eprint/972

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