Surveying brain tumor heterogeneity by single-cell RNA-sequencing of multi-sector biopsies.
Full text not available from this repository.Item Type: | Article |
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Status: | Published |
Official URL: | https://doi.org/10.1093/nsr%2Fnwaa099 |
Journal or Publication Title: | National Science Review |
Volume: | 7 |
Number: | 8 |
Page Range: | pp. 1306-1318 |
Date: | 2020 |
Divisions: | Gene and Stem Cell Therapy |
Depositing User: | General Admin |
Identification Number: | 10.1093/nsr/nwaa099 |
ISSN: | 2095-5138 |
Date Deposited: | 04 Jan 2021 02:46 |
Abstract: | Brain tumors are among the most challenging human tumors for which the mechanisms driving progression and heterogeneity remain poorly understood. We combined single-cell RNA-seq with multi-sector biopsies to sample and analyze single-cell expression profiles of gliomas from 13 Chinese patients. After classifying individual cells, we generated a spatial and temporal landscape of glioma that revealed the patterns of invasion between the different sub-regions of gliomas. We also used single-cell inferred copy number variations and pseudotime trajectories to inform on the crucial branches that dominate tumor progression. The dynamic cell components of the multi-region biopsy analysis allowed us to spatially deconvolute with unprecedented accuracy the transcriptomic features of the core and those of the periphery of glioma at single-cell level. Through this rich and geographically detailed dataset, we were also able to characterize and construct the chemokine and chemokine receptor interactions that exist among different tumor and non-tumor cells. This study provides the first spatial-level analysis of the cellular states that characterize human gliomas. It also presents an initial molecular map of the cross-talks between glioma cells and the surrounding microenvironment with single-cell resolution. |
Creators: | Creators Email Yu, Kai UNSPECIFIED Hu, Yuqiong UNSPECIFIED Wu, Fan UNSPECIFIED Guo, Qiufang UNSPECIFIED Qian, Zenghui UNSPECIFIED Hu, Waner UNSPECIFIED Chen, Jing UNSPECIFIED Wang, Kuanyu UNSPECIFIED Fan, Xiaoying UNSPECIFIED Wu, Xinglong UNSPECIFIED Rasko, John EJ UNSPECIFIED Fan, Xiaolong UNSPECIFIED Iavarone, Antonio UNSPECIFIED Jiang, Tao UNSPECIFIED Tang, Fuchou UNSPECIFIED Su, Xiao-Dong UNSPECIFIED |
Last Modified: | 04 Jan 2021 02:46 |
URI: | https://eprints.centenary.org.au/id/eprint/766 |
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