Enhancement in brain uptake of vitamin D3 nanoemulsion for treatment of cerebral ischemia: formulation, gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models

Enhancement in brain uptake of vitamin D3 nanoemulsion for treatment of cerebral ischemia: formulation, gamma scintigraphy and efficacy study in transient middle cerebral artery occlusion rat models.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1080/02652048.2020.1801870
Journal or Publication Title: Journal of Microencapsulation
Volume: 37
Number: 7
Page Range: pp. 492-501
Date: 2020
Divisions: UTS Centre for Inflammation
Depositing User: General Admin
Identification Number: 10.1080/02652048.2020.1801870
ISSN: 0265-2048
Date Deposited: 04 Jan 2021 02:51
Abstract:

Aim
For the treatment of cerebral ischaemia, vitamin-D3 loaded nanoemulsions were developed.

Method
Tween 20 and polyethylene glycol were chosen as surfactant/co-surfactant, while oleic acid as the oil phase. The formulation was characterised for various in-vitro parameters. Targeting efficiency was investigated through radiometry, gamma scintigraphy and efficacy was studied in transient middle cerebral artery occlusion (MCAo) rat model.

Result
Vitamin D3-nanoemulsion showed a mean size range of 49.29 ± 10.28 nm with polydispersity index 0.17 ± 0.04 and zeta potential 13.77 mV. The formulation was found stable during thermodynamic stability study and permeated within 180 min through sheep nasal mucosa (permeation coefficient 7.873 ± 0.884 cm/h). Gamma scintigraphy and radiometry assay confirmed better percentage deposition (2.53 ± 0.17%) of 99mTc-vitamin D3-nanoemulsion through nasal route compared to IV administered 99mTc-vitamin D3 solution (0.79 ± 0.03%). Magnetic Resonance Imaging (MRI) of the ischaemic model confirmed better efficacy of vitamin D3-nanoemulsion.

Conclusion
This work demonstrated better permeation, deposition, and efficacy of vitaminD3-nanoemulsion through the intranasal route.

Creators:
Creators
Email
Kumar, Mukesh
UNSPECIFIED
Nishad, Dhruv Kumar
UNSPECIFIED
Kumar, Anoop
UNSPECIFIED
Bhatnagar, Aseem
UNSPECIFIED
Karwasra, Ritu
UNSPECIFIED
Khanna, Kushagra
UNSPECIFIED
S, Keerthana
UNSPECIFIED
Sharma, Deeksha
UNSPECIFIED
Dua, Kamal
UNSPECIFIED
Mudaliyar, Vijaybabu
UNSPECIFIED
Sharma, Nitin
UNSPECIFIED
Last Modified: 04 Jan 2021 02:51
URI: https://eprints.centenary.org.au/id/eprint/761

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