Urinary versus serum microRNAs in human oxalic acid poisoning: Contrasting signals and performance

Urinary versus serum microRNAs in human oxalic acid poisoning: Contrasting signals and performance.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1016/j.toxlet.2020.09.003
Journal or Publication Title: Toxicology Letters
Volume: 334
Page Range: pp. 21-26
Date: 2020
Divisions: Alcoholic Liver Disease
Depositing User: General Admin
Identification Number: 10.1016/j.toxlet.2020.09.003
ISSN: 03784274
Date Deposited: 04 Jan 2021 03:13
Abstract:

MicroRNAs are key regulators of the normal kidney function and development, and altered in acute kidney injury (AKI). However, there is a lack of studies comparing serum and urine miRNA expression in toxic AKI in humans. We aimed to compare the global signature of urinary and serum microRNAs, with and without kidney injury, after human oxalic acid poisoning. We profiled urinary microRNAs in patients who ingested oxalic acid and developed no injury (No AKI n = 3), moderate injury (AKIN2 n = 3) or severe injury (AKIN3 n = 3) and healthy controls (n = 3). We validated a signature of 30 urinary microRNAs identified in the discovery profiling, in a second cohort of individuals exposed to oxalic acid (No AKI n = 15, AKIN2 n=11 & AKIN3 n= 18) and healthy controls (n=-27) and we compared the results with previously published serum data. Global profiling in toxic AKI patients showed a higher expression of urinary microRNAs and lower expression of serum microRNAs. Most urine microRNA in the validation cohort were significantly upregulated (25/30, fold change >2.8 and p < 0.05) in AKIN2/3 patients compared to No AKI. Four urinary microRNAs (miR-191, miR-19b, miR-20a and miR-30b) had good diagnostic performance (AUC greater than 0.8) to predict AKIN2/3 between 4−8 hours post ingestion. Poisoning irrespective of AKI led to significantly lower expression of many microRNAs in serum but relatively few changes in urinary miRNA expression. In conclusion, urinary microRNA signature provides a stronger measure of AKI in oxalic acid poisoning compared to serum microRNA. Kidney injury has the greatest impact on urinary microRNA, while poisoning itself was better reflected in serum miRNA. Plasma and urinary microRNAs signatures provide complementary information in toxic kidney injury.

Creators:
Creators
Email
Shihana, Fathima
UNSPECIFIED
Mohamed, Fahim
UNSPECIFIED
Joglekar, Mugdha V.
UNSPECIFIED
Hardikar, Anandwardhan A.
UNSPECIFIED
Seth, Devanshi
UNSPECIFIED
Buckley, Nicholas A.
UNSPECIFIED
Last Modified: 04 Jan 2021 03:13
URI: https://eprints.centenary.org.au/id/eprint/750

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