Rutin loaded liquid crystalline nanoparticles inhibit lipopolysaccharide induced oxidative stress and apoptosis in bronchial epithelial cells in vitro

Rutin loaded liquid crystalline nanoparticles inhibit lipopolysaccharide induced oxidative stress and apoptosis in bronchial epithelial cells in vitro.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1016/j.tiv.2020.104961
Journal or Publication Title: Toxicology in Vitro
Volume: 68
Page Range: p. 104961
Date: 2020
Divisions: UTS Centre for Inflammation
Depositing User: General Admin
Identification Number: 10.1016/j.tiv.2020.104961
ISSN: 08872333
Date Deposited: 04 Jan 2021 03:12
Abstract:

Airway inflammation and infections are the primary causes of damage in the airway epithelium, that lead to hypersecretion of mucus and airway hyper-responsiveness. The role of reactive oxygen species (ROS) and their components in the pathophysiological mechanisms of airway inflammation have been well-studied and emphasized for the past several decades. Rutin, a potent bioflavonoid, is well-known for its antioxidant, anti-inflammatory, especially in bronchial inflammation. However, poor solubility and rapid metabolism have led to its low bioavailability in biological systems, and hence limit its application. The present study aims to investigate the beneficial effects of rutin-loaded liquid crystalline nanoparticles (LCNs) against lipopolysaccharide (LPS) induced oxidative damage in human bronchial epithelial cell line (BEAS-2-B) cells in vitro. LPS was used to stimulate BEAS-2-B cells, causing the generation of nitric oxide (NO) and other reactive oxygen species (ROS) that had led to cellular apoptosis. The levels of NO and ROS were detected by, Griess reagent kit and dichlorodihydrofluorescein diacetate (DCFH-DA) respectively, whereas, cell apoptosis was studied by Annexin V-FITC and PI staining. The findings revealed that rutin-loaded LCNs significantly reduced NO, ROS levels and prevented apoptosis in BEAS-2B cells. The observations and findings provide a mechanistic understanding of the effectiveness of rutin-loaded LCNs in protecting the bronchial cells against airway inflammation, thus possessing a promising therapeutic option for the management of airway diseases.

Creators:
Creators
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Paudel, Keshav Raj
UNSPECIFIED
Wadhwa, Ridhima
UNSPECIFIED
Mehta, Meenu
UNSPECIFIED
Chellappan, Dinesh Kumar
UNSPECIFIED
Hansbro, Philip M.
UNSPECIFIED
Dua, Kamal
UNSPECIFIED
Last Modified: 04 Jan 2021 03:12
URI: https://eprints.centenary.org.au/id/eprint/749

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