Research Techniques Made Simple: Two-Photon Intravital Imaging of the Skin

Research Techniques Made Simple: Two-Photon Intravital Imaging of the Skin.

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Item Type: Article
Status: Published
Official URL:
Journal or Publication Title: Journal of Investigative Dermatology
Volume: 138
Number: 4
Page Range: pp. 720-725
Date: 2018
Divisions: Immune Imaging
Depositing User: General Admin
Identification Number: 10.1016/j.jid.2018.01.017
ISSN: 0022202X
Date Deposited: 04 Jan 2021 23:10

Over the last few years, intravital two-photon microscopy has matured into a powerful technology helping basic and clinical researchers obtain quantifiable details of complex biological mechanisms in live and intact tissues. Two-photon microscopy provides high spatial and temporal resolution in vivo with little phototoxicity that is unattainable by other optical tools like confocal microscopy. Using ultrashort laser pulses, two-photon microscopy allows the visualization of molecules, cells, and extracellular structures up to depths of 1 mm within tissues. Consequently, real-time imaging of the individual skin layers under both physiological and pathological conditions has revolutionized our understanding of cutaneous homeostasis, immunity, and tumor biology. This review provides an overview to two-photon microscopy of the skin by covering the basic concepts and current applications in diverse preclinical and clinical settings.

Obeidy, Peyman
Tong, Philip L.
Weninger, Wolfgang
Last Modified: 04 Jan 2021 23:10

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