Genomic Deletion of BAP1 and CDKN2A Are Useful Markers for Quality Control of Malignant Pleural Mesothelioma (MPM) Primary Cultures

Genomic Deletion of BAP1 and CDKN2A Are Useful Markers for Quality Control of Malignant Pleural Mesothelioma (MPM) Primary Cultures.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.3390/ijms19103056
Journal or Publication Title: International Journal of Molecular Sciences
Volume: 19
Number: 10
Page Range: p. 3056
Date: 2018
Divisions: Liver Injury and Cancer
Depositing User: General Admin
Identification Number: 10.3390/ijms19103056
ISSN: 1422-0067
Date Deposited: 04 Jan 2021 23:28
Abstract:

Malignant pleural mesothelioma (MPM) is a deadly cancer that is caused by asbestos exposure and that has limited treatment options. The current standard of MPM diagnosis requires the testing of multiple immunohistochemical (IHC) markers on formalin-fixed paraffin-embedded tissue to differentiate MPM from other lung malignancies. To date, no single biomarker exists for definitive diagnosis of MPM due to the lack of specificity and sensitivity; therefore, there is ongoing research and development in order to identify alternative biomarkers for this purpose. In this study, we utilized primary MPM cell lines and tested the expression of clinically used biomarker panels, including CK8/18, Calretinin, CK 5/6, CD141, HBME-1, WT-1, D2-40, EMA, CEA, TAG72, BG8, CD15, TTF-1, BAP1, and Ber-Ep4. The genomic alteration of CDNK2A and BAP1 is common in MPM and has potential diagnostic value. Changes in CDKN2A and BAP1 genomic expression were confirmed in MPM samples in the current study using Fluorescence In situ Hybridization (FISH) analysis or copy number variation (CNV) analysis with digital droplet PCR (ddPCR). To determine whether MPM tissue and cell lines were comparable in terms of molecular alterations, IHC marker expression was analyzed in both sample types. The percentage of MPM biomarker levels showed variation between original tissue and matched cells established in culture. Genomic deletions of BAP1 and CDKN2A, however, showed consistent levels between the two. The data from this study suggest that genomic deletion analysis may provide more accurate biomarker options for MPM diagnosis.

Creators:
Creators
Email
Sarun, Kadir
UNSPECIFIED
Lee, Kenneth
UNSPECIFIED
Williams, Marissa
UNSPECIFIED
Wright, Casey
UNSPECIFIED
Clarke, Candice
UNSPECIFIED
Cheng, Ngan
UNSPECIFIED
Takahashi, Ken
UNSPECIFIED
Cheng, Yuen
UNSPECIFIED
Last Modified: 04 Jan 2021 23:28
URI: https://eprints.centenary.org.au/id/eprint/576

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