Evaluation of a novel method for the analysis of alcohol biomarkers: Ethyl glucuronide, ethyl sulfate and phosphatidylethanol

Evaluation of a novel method for the analysis of alcohol biomarkers: Ethyl glucuronide, ethyl sulfate and phosphatidylethanol.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1016/j.alcohol.2017.08.009
Journal or Publication Title: Alcohol
Volume: 67
Page Range: pp. 7-13
Date: 2018
Divisions: Alcoholic Liver Disease
Depositing User: General Admin
Identification Number: 10.1016/j.alcohol.2017.08.009
ISSN: 07418329
Date Deposited: 03 Jan 2021 23:11

Currently available markers and methods to evaluate alcohol consumption are indirect and suboptimal, or rely on self-report, which have inherent problems. Direct metabolites of alcohol, phosphatidylethanol (PEth), ethyl sulfate (EtS), and ethyl glucuronide (EtG), are known to improve diagnostic accuracy. In this study, methods were established for the identification of PEth in erythrocytes and EtG and EtS in serum using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The three biomarkers were tested and validated in volunteer teetotalers (n = 4) and drinkers (n = 10), and applied in patients (n = 8) hospitalized with alcohol-related problems. Linearity of each assay was demonstrated from 22.5 to 900 nM for EtG, 40-3175 nM for EtS, and 21-750 nM for PEth. The methods were highly selective, precise (<5% coefficient of variation), and had optimal accuracy (within 10% of the nominal value) for all three analytes. Recovery for all three compounds exceeded 90%. A preliminary investigation into the window of detection of these biomarkers after a single occasion of moderate alcohol consumption revealed that EtG and EtS could be detected and quantified over the short term (days) and PEth over the long term (weeks). All three biomarkers showed high sensitivity and specificity in distinguishing between abstinence and any alcohol use at the cut-off values of 22.5 nM for EtG, 40 nM for EtS, and 21 nM for PEth. We have established simultaneous assays for EtG, EtS, and PEth for routine clinical use in confirming abstinence and exposure, and detecting under-reporting of alcohol use, relevant in clinical and non-clinical settings.

Keywords: Alcohol-use biomarker; Self-report; UHPLC-Tandem mass spectrometry.

Crown Copyright © 2017. Published by Elsevier Inc. All rights reserved.

Nguyen, Van Long
Paull, Phillip
Haber, Paul S.
Chitty, Kate
Seth, Devanshi
Last Modified: 03 Jan 2021 23:11
URI: https://eprints.centenary.org.au/id/eprint/481

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