The impact of ischemia‐reperfusion injuries on skin resident murine dendritic cells

The impact of ischemia‐reperfusion injuries on skin resident murine dendritic cells.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1002/eji.201747347
Journal or Publication Title: European Journal of Immunology
Volume: 48
Number: 6
Page Range: pp. 1014-1019
Date: 2018
Subjects: Fields of Research > 11 MEDICAL AND HEALTH SCIENCES > 1116 MEDICAL PHYSIOLOGY > 111601 Cell Physiology
11 MEDICAL AND HEALTH SCIENCES > 1116 MEDICAL PHYSIOLOGY > 111601 Cell Physiology
Divisions: Immune Imaging
Depositing User: General Admin
Identification Number: 10.1002/eji.201747347
ISSN: 0014-2980
Date Deposited: 17 Dec 2020 03:37
Abstract:

Pressure ulcers are a chronic problem for patients or the elderly who require extended periods of bed rest. The formation of ulcers is due to repeated cycles of ischemia-reperfusion (IR), which initiates an inflammatory response. Advanced ulcers disrupt the skin barrier, resulting in further complications. To date, the immunological aspect of skin IR has been understudied, partly due to the complexity of the skin immune cells. Through a combination of mass cytometry, confocal imaging and intravital multiphoton imaging, this study establishes a workflow for multidimensionality single cell analysis of skin myeloid cell responses in the context of IR injury with high spatiotemporal resolution. The data generated has provided us with previously uncharacterized insights into the distinct cellular behavior of resident dendritic cells (DCs) and recruited neutrophils post IR. Of interest, we observed a drop in DDC numbers in the IR region, which was subsequently replenished 48h post IR. More importantly, in these cells, we observe an attenuated response to repeated injuries, which may have implications in the subsequent wound healing process.

Creators:
Creators
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Goh, Chi Ching
UNSPECIFIED
Evrard, Maximilien
UNSPECIFIED
Chong, Shu Zhen
UNSPECIFIED
Tan, Yingrou
UNSPECIFIED
Tan, Leonard De Li
UNSPECIFIED
Teng, Karen Wei Weng
UNSPECIFIED
Weninger, Wolfgang
UNSPECIFIED
Becker, David Laurence
UNSPECIFIED
Tey, Hong Liang
UNSPECIFIED
Newell, Evan William
UNSPECIFIED
Liu, Bin
UNSPECIFIED
Ng, Lai Guan
UNSPECIFIED
Last Modified: 17 Dec 2020 03:37
URI: https://eprints.centenary.org.au/id/eprint/471

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