DNA methylation/hydroxymethylation regulate gene expression and alternative splicing during terminal granulopoiesis.
Full text not available from this repository.Item Type: | Article |
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Status: | Published |
Official URL: | https://doi.org/10.2217/epi-2018-0050 |
Journal or Publication Title: | Epigenomics |
Volume: | 11 |
Number: | 1 |
Page Range: | pp. 95-109 |
Date: | 2019 |
Divisions: | Epigenetics and RNA Biology Gene and Stem Cell Therapy |
Depositing User: | General Admin |
Identification Number: | 10.2217/epi-2018-0050 |
ISSN: | 1750-1911 |
Date Deposited: | 21 Dec 2020 05:53 |
Abstract: | Aim: To determine whether epigenetic modifications of DNA regulate gene expression and alternative splicing during terminal granulopoiesis. Materials & methods: Using whole genome bisulfite sequencing, reduced representation hydroxymethylation profiling and mRNA sequencing, we compare changes in DNA methylation, DNA hydroxymethylation, gene expression and alternative splicing in mouse promyelocytes and granulocytes. Results & conclusion: We show reduced DNA methylation at the promoters and enhancers of key granulopoiesis genes, indicating a regulatory role in the activation of lineage-specific genes during differentiation. Notably, increased DNA hydroxymethylation in exons is associated with preferential inclusion of specific exons in granulocytes. Overall, DNA methylation and hydroxymethylation changes at particular genomic loci may play specific roles in gene regulation or alternative splicing during terminal granulopoiesis. Data deposition: Whole genome bisulfite sequencing of mouse promyelocytes and granulocytes: Gene Expression Omnibus (GSE85517); mRNA sequencing of mouse promyelocytes and granulocytes: Gene Expression Omnibus (GSE48307); reduced representation 5-hydroxymethylation profiling of mouse promyelocytes and granulocytes: Bioproject (PRJNA495696). Keywords: DNA hydroxymethylation; DNA methylation; alternative splicing; gene expression; granulopoiesis; mRNA sequencing; whole genome bisulfite sequencing. |
Creators: | Creators Email Gao, Dadi UNSPECIFIED Pinello, Natalia UNSPECIFIED Nguyen, Trung V UNSPECIFIED Thoeng, Annora UNSPECIFIED Nagarajah, Rajini UNSPECIFIED Holst, Jeff UNSPECIFIED Rasko, John EJ UNSPECIFIED Wong, Justin J-L UNSPECIFIED |
Last Modified: | 21 Dec 2020 05:53 |
URI: | https://eprints.centenary.org.au/id/eprint/317 |
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