DNA methylation/hydroxymethylation regulate gene expression and alternative splicing during terminal granulopoiesis

DNA methylation/hydroxymethylation regulate gene expression and alternative splicing during terminal granulopoiesis.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.2217/epi-2018-0050
Journal or Publication Title: Epigenomics
Volume: 11
Number: 1
Page Range: pp. 95-109
Date: 2019
Divisions: Epigenetics and RNA Biology
Gene and Stem Cell Therapy
Depositing User: General Admin
Identification Number: 10.2217/epi-2018-0050
ISSN: 1750-1911
Date Deposited: 21 Dec 2020 05:53
Abstract:

Aim: To determine whether epigenetic modifications of DNA regulate gene expression and alternative splicing during terminal granulopoiesis.

Materials & methods: Using whole genome bisulfite sequencing, reduced representation hydroxymethylation profiling and mRNA sequencing, we compare changes in DNA methylation, DNA hydroxymethylation, gene expression and alternative splicing in mouse promyelocytes and granulocytes.

Results & conclusion: We show reduced DNA methylation at the promoters and enhancers of key granulopoiesis genes, indicating a regulatory role in the activation of lineage-specific genes during differentiation. Notably, increased DNA hydroxymethylation in exons is associated with preferential inclusion of specific exons in granulocytes. Overall, DNA methylation and hydroxymethylation changes at particular genomic loci may play specific roles in gene regulation or alternative splicing during terminal granulopoiesis. Data deposition: Whole genome bisulfite sequencing of mouse promyelocytes and granulocytes: Gene Expression Omnibus (GSE85517); mRNA sequencing of mouse promyelocytes and granulocytes: Gene Expression Omnibus (GSE48307); reduced representation 5-hydroxymethylation profiling of mouse promyelocytes and granulocytes: Bioproject (PRJNA495696).

Keywords: DNA hydroxymethylation; DNA methylation; alternative splicing; gene expression; granulopoiesis; mRNA sequencing; whole genome bisulfite sequencing.

Creators:
Creators
Email
Gao, Dadi
UNSPECIFIED
Pinello, Natalia
UNSPECIFIED
Nguyen, Trung V
UNSPECIFIED
Thoeng, Annora
UNSPECIFIED
Nagarajah, Rajini
UNSPECIFIED
Holst, Jeff
UNSPECIFIED
Rasko, John EJ
UNSPECIFIED
Wong, Justin J-L
UNSPECIFIED
Last Modified: 21 Dec 2020 05:53
URI: https://eprints.centenary.org.au/id/eprint/317

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