Spred negatively regulates lens growth by modulating epithelial cell proliferation and fiber differentiation.
Full text not available from this repository.Item Type: | Article |
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Status: | Published |
Official URL: | https://doi.org/10.1016/j.exer.2018.09.019 |
Journal or Publication Title: | Experimental Eye Research |
Volume: | 178 |
Page Range: | pp. 160-175 |
Date: | 2019 |
Divisions: | Gene and Stem Cell Therapy |
Depositing User: | General Admin |
Identification Number: | 10.1016/j.exer.2018.09.019 |
ISSN: | 00144835 |
Date Deposited: | 21 Dec 2020 05:54 |
Abstract: | Spred, like Sprouty (Spry) and also Sef proteins, have been identified as important regulators of receptor tyrosine kinase (RTK)-mediated MAPK/ERK-signaling in various developmental systems, controlling cellular processes such as proliferation, migration and differentiation. Spreds are widely expressed during early embryogenesis, and in the eye lens, become more localised in the lens epithelium with later development, overlapping with other antagonists including Spry. Given the synexpression of Spreds and Spry in lens, in order to gain a better understanding of their specific roles in regulating growth factor mediated-signaling and cell behavior, we established and characterised lines of transgenic mice overexpressing Spred1 or Spred2, specifically in the lens. This overexpression of Spreds resulted in a small lens phenotype during ocular morphogenesis, retarding its growth by compromising epithelial cell proliferation and fiber differentiation. These in situ findings were shown to be dependent on the ability of Spreds to suppress MAPK-signaling, in particular FGF-induced ERK1/2-signaling in lens cells. This was validated in vitro using lens epithelial explants, that highlighted the overlapping role of Spreds with Spry2, but not Spry1. This study provides insights into the putative function of Spreds and Spry in situ, some overlapping and some distinct, and their importance in regulating lens cell proliferation and fiber differentiation contributing to lens and eye growth. Keywords: Development; ERK1/2; FGF; Fiber differentiation; Lens epithelial cell proliferation; Spred; Sprouty. Crown Copyright © 2018. Published by Elsevier Ltd. All rights reserved. |
Creators: | Creators Email Susanto, Alyssa UNSPECIFIED Zhao, Guannan UNSPECIFIED Wazin, Fatima UNSPECIFIED Feng, Yue UNSPECIFIED Rasko, John E.J. UNSPECIFIED Bailey, Charles G. UNSPECIFIED Lovicu, Frank J. UNSPECIFIED |
Last Modified: | 21 Dec 2020 05:54 |
URI: | https://eprints.centenary.org.au/id/eprint/315 |
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