Novel role of extracellular matrix protein 1 (ECM1) in cardiac aging and myocardial infarction

Novel role of extracellular matrix protein 1 (ECM1) in cardiac aging and myocardial infarction.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1371/journal.pone.0212230
Journal or Publication Title: PLOS ONE
Volume: 14
Number: 2
Page Range: e0212230
Date: 2019
Divisions: UTS Centre for Inflammation
Depositing User: General Admin
Identification Number: 10.1371/journal.pone.0212230
ISSN: 1932-6203
Date Deposited: 17 Dec 2020 02:52
Abstract:

Introduction: The prevalence of heart failure increases in the aging population and following myocardial infarction (MI), yet the extracellular matrix (ECM) remodeling underpinning the development of aging- and MI-associated cardiac fibrosis remains poorly understood. A link between inflammation and fibrosis in the heart has long been appreciated, but has mechanistically remained undefined. We investigated the expression of a novel protein, extracellular matrix protein 1 (ECM1) in the aging and infarcted heart.

Methods: Young adult (3-month old) and aging (18-month old) C57BL/6 mice were assessed. Young mice were subjected to left anterior descending artery-ligation to induce MI, or transverse aortic constriction (TAC) surgery to induce pressure-overload cardiomyopathy. Left ventricle (LV) tissue was collected early and late post-MI/TAC. Bone marrow cells (BMCs) were isolated from young healthy mice, and subject to flow cytometry. Human cardiac fibroblast (CFb), myocyte, and coronary artery endothelial & smooth muscle cell lines were cultured; human CFbs were treated with recombinant ECM1. Primary mouse CFbs were cultured and treated with recombinant angiotensin-II or TGF-β1. Immunoblotting, qPCR and mRNA fluorescent in-situ hybridization (mRNA-FISH) were conducted on LV tissue and cells.

Results: ECM1 expression was upregulated in the aging LV, and in the infarct zone of the LV early post-MI. No significant differences in ECM1 expression were found late post-MI or at any time-point post-TAC. ECM1 was not expressed in any resident cardiac cells, but ECM1 was highly expressed in BMCs, with high ECM1 expression in granulocytes. Flow cytometry of bone marrow revealed ECM1 expression in large granular leucocytes. mRNA-FISH revealed that ECM1 was indeed expressed by inflammatory cells in the infarct zone at day-3 post-MI. ECM1 stimulation of CFbs induced ERK1/2 and AKT activation and collagen-I expression, suggesting a pro-fibrotic role.

Conclusions: ECM1 expression is increased in ageing and infarcted hearts but is not expressed by resident cardiac cells. Instead it is expressed by bone marrow-derived granulocytes. ECM1 is sufficient to induce cardiac fibroblast stimulation in vitro. Our findings suggest ECM1 is released from infiltrating inflammatory cells, which leads to cardiac fibroblast stimulation and fibrosis in aging and MI. ECM1 may be a novel intermediary between inflammation and fibrosis.

Creators:
Creators
Email
Singla, Dinender K.
UNSPECIFIED
Hardy, Sean A.
UNSPECIFIED
Mabotuwana, Nishani S.
UNSPECIFIED
Murtha, Lucy A.
UNSPECIFIED
Coulter, Brianna
UNSPECIFIED
Sanchez-Bezanilla, Sonia
UNSPECIFIED
Al-Omary, Mohammed S.
UNSPECIFIED
Senanayake, Tharindu
UNSPECIFIED
Loering, Svenja
UNSPECIFIED
Starkey, Malcolm
UNSPECIFIED
Lee, Randall J.
UNSPECIFIED
Rainer, Peter P.
UNSPECIFIED
Hansbro, Philip M.
UNSPECIFIED
Boyle, Andrew J.
UNSPECIFIED
Last Modified: 17 Dec 2020 02:52
URI: https://eprints.centenary.org.au/id/eprint/279

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