Storage stability of inhalable phage powders containing lactose at ambient conditions.
Full text not available from this repository.Item Type: | Article |
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Status: | Published |
Official URL: | https://doi.org/10.1016/j.ijpharm.2019.01.050 |
Journal or Publication Title: | International Journal of Pharmaceutics |
Volume: | 560 |
Page Range: | pp. 11-18 |
Date: | 2019 |
Divisions: | Tuberculosis |
Depositing User: | General Admin |
Identification Number: | 10.1016/j.ijpharm.2019.01.050 |
ISSN: | 03785173 |
Date Deposited: | 21 Dec 2020 06:01 |
Abstract: | The aim of this study was to evaluate the storage stability of inhalable phage powders containing lactose and leucine as excipient. As an FDA-approved excipient for inhalation, lactose is preferred over other sugars. PEV phages active against antibiotic-resistant Pseudomonas aeruginosa was spray dried with lactose (55-90%) and leucine (45-10%). Produced powders were heat-sealed in an aluminium pouch at 15% relative humidity (RH) with subsequent storage at 20 °C/60% RH for 12 months. Lactose concentration in the powder positively influenced the phage stability over time. Formulation containing 90% lactose maintained the viability of PEV61 across the study, while ∼1.2 log10 titer reduction was observed in formulations with less lactose. PEV20 was more prone to inactivation (1.7 log10 titer loss at 12-month) when lactose concentration in the particle was below 80%. The fine particle fraction (% wt. particles <5 μm in aerosol) of phage powders was 52-61% and remained the same after 12-month storage. The results demonstrate that spray dried PEV phage powders containing lactose and leucine are biologically and physically stable over long-term storage at ambient temperature. Furthermore, these spray dried phage powders were shown to be non-toxic to lung alveolar macrophage and epithelial cells in vitro. Keywords: Bacteriophage (Phage); Inhalation aerosol; Lactose; Spray dried powders; Storage stability. Copyright © 2019 Elsevier B.V. All rights reserved. |
Creators: | Creators Email Chang, Rachel Yoon Kyung UNSPECIFIED Wallin, Martin UNSPECIFIED Kutter, Elizabeth UNSPECIFIED Morales, Sandra UNSPECIFIED Britton, Warwick UNSPECIFIED Li, Jian UNSPECIFIED Chan, Hak-Kim UNSPECIFIED |
Last Modified: | 21 Dec 2020 06:01 |
URI: | https://eprints.centenary.org.au/id/eprint/240 |
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