Non-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins

Non-canonical translation initiation in yeast generates a cryptic pool of mitochondrial proteins.

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Item Type: Article
Status: Published
Official URL:
Journal or Publication Title: Nucleic Acids Research
Volume: 47
Number: 11
Page Range: pp. 5777-5791
Date: 2019
Divisions: Gene and Stem Cell Therapy
Depositing User: General Admin
Identification Number: 10.1093/nar/gkz301
ISSN: 0305-1048
Date Deposited: 21 Dec 2020 22:58

Utilization of non-AUG alternative translation start sites is most common in bacteria and viruses, but it has been also reported in other organisms. This phenomenon increases proteome complexity by allowing expression of multiple protein isoforms from a single gene. In Saccharomyces cerevisiae, a few described cases concern proteins that are translated from upstream near-cognate start codons as N-terminally extended variants that localize to mitochondria. Using bioinformatics tools, we provide compelling evidence that in yeast the potential for producing alternative protein isoforms by non-AUG translation initiation is much more prevalent than previously anticipated and may apply to as many as a few thousand proteins. Several hundreds of candidates are predicted to gain a mitochondrial targeting signal (MTS), generating an unrecognized pool of mitochondrial proteins. We confirmed mitochondrial localization of a subset of proteins previously not identified as mitochondrial, whose standard forms do not carry an MTS. Our data highlight the potential of non-canonical translation initiation in expanding the capacity of the mitochondrial proteome and possibly also other cellular features.

© The Author(s) 2019. Published by Oxford University Press on behalf of Nucleic Acids Research.

Monteuuis, Geoffray
Miścicka, Anna
Świrski, Michał
Zenad, Lounis
Niemitalo, Olli
Wrobel, Lidia
Alam, Jahangir
Chacinska, Agnieszka
Kastaniotis, Alexander J
Kufel, Joanna
Last Modified: 21 Dec 2020 22:58

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