Mapping the mutual transcriptional responses during Candida albicans and human macrophage interactions by dual RNA-sequencing

Mapping the mutual transcriptional responses during Candida albicans and human macrophage interactions by dual RNA-sequencing.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1016/j.micpath.2022.105864
Journal or Publication Title: Microbial Pathogenesis
Volume: 173
Page Range: p. 105864
Date: 4 November 2022
Divisions: Gene and Stem Cell Therapy
Depositing User: General Admin
Identification Number: 10.1016/j.micpath.2022.105864
ISSN: 08824010
Date Deposited: 15 Mar 2023 00:42
Abstract:

Candida albicans is the leading human fungal pathogen that can cause mucosal and systemic fungal infections. Host phagocytes are the primary immune defense against invading fungal pathogens including C. albicans. To better understand the host-pathogen interaction between C. albicans and host phagocytes, we utilized a human macrophage model of THP-1 macrophages and examined the mutual transcriptomic response of C. albicans and host macrophages by dual RNA-sequencing. Both C. albicans and macrophages displayed marked changes in their transcriptional profiles post 2 h coincubation. We show that C. albicans responds to human macrophages differently than its known response to murine macrophages. C. albicans displays upregulation of its translational machinery and downregulation of glyoxylate and tricarboxylic acid (TCA) cycle upon macrophage phagocytosis. C. albicans triggered strong induction of genes associated with cell surface-mediated signaling and proinflammatory response in THP-1 macrophages. Finally, our data reveal that IL-1β and TNF signaling are central in mounting a proinflammatory response against C. albicans via MAP kinase, and chemokines and cytokines mediated signaling. Overall, current work uncovers the mutual responses of C. albicans and human macrophages towards each other presenting a better understanding of their interaction during C. albicans infections.

Creators:
Creators
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Rai, Maruti Nandan
UNSPECIFIED
Parsania, Chirag
UNSPECIFIED
Rai, Rikky
UNSPECIFIED
Last Modified: 15 Mar 2023 00:42
URI: https://eprints.centenary.org.au/id/eprint/1438

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