Illuminating the dark protein-protein interactome

Illuminating the dark protein-protein interactome.

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Item Type: Article
Status: Published
Official URL:
Journal or Publication Title: Cell Reports Methods
Volume: 2
Number: 8
Page Range: p. 100275
Date: 22 August 2022
Divisions: Gene and Stem Cell Therapy
Depositing User: General Admin
Identification Number: 10.1016/j.crmeth.2022.100275
ISSN: 26672375
Date Deposited: 15 Mar 2023 04:32

In living systems, a complex network of protein-protein interactions (PPIs) underlies most biochemical events. The human protein-protein interactome has been surveyed using yeast two-hybrid (Y2H)- and mass spectrometry (MS)-based approaches such as affinity purification coupled to MS (AP-MS). Despite decades of systematic investigations and collaborative multi-disciplinary efforts, there is no "gold standard" for documenting PPIs. A surprisingly large fraction of the human interactome remains uncharted, which we refer to as the "dark interactome." In this review, we highlight the complexity of the human interactome and discuss the current status of the human reference interactome maps. We discuss why a large proportion of the human interactome has remained refractory to traditional approaches. We propose an experimental model that can enable the identification of the dark interactome in a cell-type-specific manner. We also propose a framework to implement when embarking on studies designed to rigorously identify and characterize protein interactions.

Sharifi Tabar, Mehdi
Parsania, Chirag
Chen, Hong
Su, Xiao-Dong
Bailey, Charles G.
Rasko, John E.J.
Last Modified: 15 Mar 2023 04:32

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