Navigating the cellular landscape in tissue: Recent advances in defining the pathogenesis of human disease

Navigating the cellular landscape in tissue: Recent advances in defining the pathogenesis of human disease.

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Item Type: Review
Status: Published
Official URL:
Journal or Publication Title: Computational and Structural Biotechnology Journal
Volume: 20
Page Range: pp. 5256-5263
Date: 2022
Divisions: Human Viral and Cancer Immunology
Depositing User: General Admin
Identification Number: 10.1016/j.csbj.2022.09.005
ISSN: 20010370
Date Deposited: 17 Apr 2023 00:34

Over the past decade, our understanding of human diseases has rapidly grown from the rise of single-cell spatial biology. While conventional tissue imaging has focused on visualizing morphological features, the development of multiplex tissue imaging from fluorescence-based methods to DNA- and mass cytometry-based methods has allowed visualization of over 60 markers on a single tissue section. The advancement of spatial biology with a single-cell resolution has enabled the visualization of cell–cell interactions and the tissue microenvironment, a crucial part to understanding the mechanisms underlying pathogenesis. Alongside the development of extensive marker panels which can distinguish distinct cell phenotypes, multiplex tissue imaging has facilitated the analysis of high dimensional data to identify novel biomarkers and therapeutic targets, while considering the spatial context of the cellular environment. This mini-review provides an overview of the recent advancements in multiplex imaging technologies and examines how these methods have been used in exploring pathogenesis and biomarker discovery in cancer, autoimmune and infectious diseases.

Chen, Helen Y.
Palendira, Umaimainthan
Feng, Carl G.
Last Modified: 17 Apr 2023 00:34

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