Preparation, characterization and in-vitro efficacy of quercetin loaded liquid crystalline nanoparticles for the treatment of asthma

Preparation, characterization and in-vitro efficacy of quercetin loaded liquid crystalline nanoparticles for the treatment of asthma.

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Item Type: Article
Official URL: https://doi.org/10.1016/j.jddst.2019.101297
Journal or Publication Title: Journal of Drug Delivery Science and Technology
Volume: 54
Page Range: p. 101297
Date: 2019
Divisions: UTS Centre for Inflammation
Depositing User: General Admin
Identification Number: 10.1016/j.jddst.2019.101297
ISSN: 17732247
Date Deposited: 22 Dec 2020 02:51
Abstract:

The present study aims to formulate quercetin loaded liquid crystalline nanoparticles (LCN) and surface modified liquid crystalline nanoparticles (sm-LCN) as well as investigate their anti-inflammatory activity in human primary bronchial epithelial cell line (BCi-NS1.1) induced with lipopolysaccharide (LPS). Quercetin LCN were prepared using ultrasonication method. The formulated LCNs and sm-LCNs were characterised in terms of particle size, zeta potential as well as the drug encapsulation efficiency. Furthermore, their morphology and in vitro release profile were also studied. In addition, the anti-inflammatory activity of quercetin LCN and sm-LCNs were evaluated by measuring the concentration of pro-inflammatory markers namely interleukin (IL)-1β, IL-6 and IL-8 in BCI-NS1.1 cell lines via cytometric bead array. The molecular mechanism inherent to the inclusion of quercetin into monoolein nanosystem and surface modification of the nanosystem with chitosan was elucidated via molecular mechanics simulations. Quercetin LCN and sm-LCN significantly (p < 0.05) decreased the production of IL-1β, IL-6 and IL-8 compared to LPS only group. Encapsulation of quercetin into LCN and sm-LCN further enhanced its anti-inflammatory activity compared to quercetin in dimethyl sulfoxide (DMSO). In addition to that, quercetin LCN and sm-LCN also exhibited comparable activity to fluticasone in terms of significantly (p < 0.05) reducing the production of IL-1β and IL-6. Quercetin loaded LCN and sm-LCN could be a potential therapeutic intervention for asthma as they are efficacious in suppressing the production of key pro-inflammatory cytokines associated with the development of asthma.

Creators:
Creators
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Cherk Yong, David Ong
UNSPECIFIED
Saker, Sanggetha Raja
UNSPECIFIED
Wadhwa, Ridhima
UNSPECIFIED
Chellappan, Dinesh Kumar
UNSPECIFIED
Madheswaran, Thiagarajan
UNSPECIFIED
Panneerselvam, Jithendra
UNSPECIFIED
Tambuwala, Murtaza M.
UNSPECIFIED
Bakshi, Hamid A.
UNSPECIFIED
Kumar, Pradeep
UNSPECIFIED
Pillay, Viness
UNSPECIFIED
Gupta, Gaurav
UNSPECIFIED
Oliver, Brian G.
UNSPECIFIED
Wark, Peter
UNSPECIFIED
Hsu, Alan
UNSPECIFIED
Hansbro, Philip Michael
UNSPECIFIED
Dua, Kamal
UNSPECIFIED
Zeeshan, Farrukh
UNSPECIFIED
Last Modified: 22 Dec 2020 02:51
URI: https://eprints.centenary.org.au/id/eprint/130

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