Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration

Zebrafish Heme Oxygenase 1a Is Necessary for Normal Development and Macrophage Migration.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1089/zeb.2021.0058
Journal or Publication Title: Zebrafish
Volume: 19
Number: 1
Page Range: pp. 7-17
Date: 2022
Divisions: Tuberculosis
Depositing User: General Admin
Identification Number: 10.1089/zeb.2021.0058
ISSN: 1545-8547
Date Deposited: 01 Apr 2022 00:18

Heme oxygenase function is highly conserved between vertebrates where it plays important roles in normal embryonic development and controls oxidative stress. Expression of the zebrafish heme oxygenase 1 genes is known to be responsive to oxidative stress suggesting a conserved physiological function. In this study, we generate a knockout allele of zebrafish hmox1a and characterize the effects of hmox1a and hmox1b loss on embryonic development. We find that loss of hmox1a or hmox1b causes developmental defects in only a minority of embryos, in contrast to Hmox1 gene deletions in mice that cause loss of most embryos. Using a tail wound inflammation assay we find a conserved role for hmox1a, but not hmox1b, in normal macrophage migration to the wound site. Together our results indicate that zebrafish hmox1a has clearly a partitioned role from hmox1b that is more consistent with conserved functions of mammalian Heme oxygenase 1.

Luo, Kaiming
Ogawa, Masahito
Ayer, Anita
Britton, Warwick J.
Stocker, Roland
Kikuchi, Kazu
Oehlers, Stefan H.
Last Modified: 01 Apr 2022 00:18
URI: https://eprints.centenary.org.au/id/eprint/1217

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