A single dose, BCG-adjuvanted COVID-19 vaccine provides sterilising immunity against SARS-CoV-2 infection

A single dose, BCG-adjuvanted COVID-19 vaccine provides sterilising immunity against SARS-CoV-2 infection.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1038/s41541-021-00406-4
Journal or Publication Title: npj Vaccines
Volume: 6
Number: 1
Date: 2021
Divisions: Human Viral and Cancer Immunology
Tuberculosis
UTS Centre for Inflammation
Depositing User: General Admin
Identification Number: 10.1038/s41541-021-00406-4
ISSN: 2059-0105
Date Deposited: 17 Mar 2022 01:21
Abstract:

Global control of COVID-19 requires broadly accessible vaccines that are effective against SARS-CoV-2 variants. In this report, we exploit the immunostimulatory properties of bacille Calmette-Guérin (BCG), the existing tuberculosis vaccine, to deliver a vaccination regimen with potent SARS-CoV-2-specific protective immunity. Combination of BCG with a stabilised, trimeric form of SARS-CoV-2 spike antigen promoted rapid development of virus-specific IgG antibodies in the blood of vaccinated mice, that was further augmented by the addition of alum. This vaccine formulation, BCG:CoVac, induced high-titre SARS-CoV-2 neutralising antibodies (NAbs) and Th1-biased cytokine release by vaccine-specific T cells, which correlated with the early emergence of T follicular helper cells in local lymph nodes and heightened levels of antigen-specific plasma B cells after vaccination. Vaccination of K18-hACE2 mice with a single dose of BCG:CoVac almost completely abrogated disease after SARS-CoV-2 challenge, with minimal inflammation and no detectable virus in the lungs of infected animals. Boosting BCG:CoVac-primed mice with a heterologous vaccine further increased SARS-CoV-2-specific antibody responses, which effectively neutralised B.1.1.7 and B.1.351 SARS-CoV-2 variants of concern. These findings demonstrate the potential for BCG-based vaccination to protect against major SARS-CoV-2 variants circulating globally.

Creators:
Creators
Email
Counoupas, Claudio
UNSPECIFIED
Johansen, Matt D.
UNSPECIFIED
Stella, Alberto O.
UNSPECIFIED
Nguyen, Duc H.
UNSPECIFIED
Ferguson, Angela L.
UNSPECIFIED
Aggarwal, Anupriya
UNSPECIFIED
Bhattacharyya, Nayan D.
UNSPECIFIED
Grey, Alice
UNSPECIFIED
Hutchings, Owen
UNSPECIFIED
Patel, Karishma
UNSPECIFIED
Siddiquee, Rezwan
UNSPECIFIED
Stewart, Erica L.
UNSPECIFIED
Feng, Carl G.
UNSPECIFIED
Hansbro, Nicole G.
UNSPECIFIED
Palendira, Umaimainthan
UNSPECIFIED
Steain, Megan C.
UNSPECIFIED
Saunders, Bernadette M.
UNSPECIFIED
Low, Jason K. K.
UNSPECIFIED
Mackay, Joel P.
UNSPECIFIED
Kelleher, Anthony D.
UNSPECIFIED
Britton, Warwick J.
UNSPECIFIED
Turville, Stuart G.
UNSPECIFIED
Hansbro, Philip M.
UNSPECIFIED
Triccas, James A.
UNSPECIFIED
Last Modified: 17 Mar 2022 01:21
URI: https://eprints.centenary.org.au/id/eprint/1198

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