A single dose, BCG-adjuvanted COVID-19 vaccine provides sterilising immunity against SARS-CoV-2 infection.
Full text not available from this repository.Item Type: | Article |
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Status: | Published |
Official URL: | https://doi.org/10.1038/s41541-021-00406-4 |
Journal or Publication Title: | npj Vaccines |
Volume: | 6 |
Number: | 1 |
Date: | 2021 |
Divisions: | Human Viral and Cancer Immunology Tuberculosis UTS Centre for Inflammation |
Depositing User: | General Admin |
Identification Number: | 10.1038/s41541-021-00406-4 |
ISSN: | 2059-0105 |
Date Deposited: | 17 Mar 2022 01:21 |
Abstract: | Global control of COVID-19 requires broadly accessible vaccines that are effective against SARS-CoV-2 variants. In this report, we exploit the immunostimulatory properties of bacille Calmette-Guérin (BCG), the existing tuberculosis vaccine, to deliver a vaccination regimen with potent SARS-CoV-2-specific protective immunity. Combination of BCG with a stabilised, trimeric form of SARS-CoV-2 spike antigen promoted rapid development of virus-specific IgG antibodies in the blood of vaccinated mice, that was further augmented by the addition of alum. This vaccine formulation, BCG:CoVac, induced high-titre SARS-CoV-2 neutralising antibodies (NAbs) and Th1-biased cytokine release by vaccine-specific T cells, which correlated with the early emergence of T follicular helper cells in local lymph nodes and heightened levels of antigen-specific plasma B cells after vaccination. Vaccination of K18-hACE2 mice with a single dose of BCG:CoVac almost completely abrogated disease after SARS-CoV-2 challenge, with minimal inflammation and no detectable virus in the lungs of infected animals. Boosting BCG:CoVac-primed mice with a heterologous vaccine further increased SARS-CoV-2-specific antibody responses, which effectively neutralised B.1.1.7 and B.1.351 SARS-CoV-2 variants of concern. These findings demonstrate the potential for BCG-based vaccination to protect against major SARS-CoV-2 variants circulating globally. |
Creators: | Creators Email Counoupas, Claudio UNSPECIFIED Johansen, Matt D. UNSPECIFIED Stella, Alberto O. UNSPECIFIED Nguyen, Duc H. UNSPECIFIED Ferguson, Angela L. UNSPECIFIED Aggarwal, Anupriya UNSPECIFIED Bhattacharyya, Nayan D. UNSPECIFIED Grey, Alice UNSPECIFIED Hutchings, Owen UNSPECIFIED Patel, Karishma UNSPECIFIED Siddiquee, Rezwan UNSPECIFIED Stewart, Erica L. UNSPECIFIED Feng, Carl G. UNSPECIFIED Hansbro, Nicole G. UNSPECIFIED Palendira, Umaimainthan UNSPECIFIED Steain, Megan C. UNSPECIFIED Saunders, Bernadette M. UNSPECIFIED Low, Jason K. K. UNSPECIFIED Mackay, Joel P. UNSPECIFIED Kelleher, Anthony D. UNSPECIFIED Britton, Warwick J. UNSPECIFIED Turville, Stuart G. UNSPECIFIED Hansbro, Philip M. UNSPECIFIED Triccas, James A. UNSPECIFIED |
Last Modified: | 17 Mar 2022 01:21 |
URI: | https://eprints.centenary.org.au/id/eprint/1198 |
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