Adaptation to an amoeba host drives selection of virulence-associated traits in Vibrio cholerae

Adaptation to an amoeba host drives selection of virulence-associated traits in Vibrio cholerae.

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Item Type: Article
Status: Published
Official URL: https://doi.org/10.1038/s41396-021-01134-2
Journal or Publication Title: The ISME Journal
Date: 15 October 2021
Divisions: Immune-Vascular Interactions
Depositing User: General Admin
Identification Number: 10.1038/s41396-021-01134-2
ISSN: 1751-7362
Date Deposited: 07 Dec 2021 09:48
Abstract:

Predation by heterotrophic protists drives the emergence of adaptive traits in bacteria, and often these traits lead to altered interactions with hosts and persistence in the environment. Here we studied adaptation of the cholera pathogen, Vibrio cholerae during long-term co-incubation with the protist host, Acanthamoeba castellanii. We determined phenotypic and genotypic changes associated with long-term intra-amoebal host adaptation and how this impacts pathogen survival and fitness. We showed that adaptation to the amoeba host leads to temporal changes in multiple phenotypic traits in V. cholerae that facilitate increased survival and competitive fitness in amoeba. Genome sequencing and mutational analysis revealed that these altered lifestyles were linked to non-synonymous mutations in conserved regions of the flagellar transcriptional regulator, flrA. Additionally, the mutations resulted in enhanced colonisation in zebrafish, establishing a link between adaptation of V. cholerae to amoeba predation and enhanced environmental persistence. Our results show that pressure imposed by amoeba on V. cholerae selects for flrA mutations that serves as a key driver for adaptation. Importantly, this study provides evidence that adaptive traits that evolve in pathogens in response to environmental predatory pressure impact the colonisation of eukaryotic organisms by these pathogens.

© 2021. The Author(s).

Creators:
Creators
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Hoque, M. Mozammel
UNSPECIFIED
Noorian, Parisa
UNSPECIFIED
Espinoza-Vergara, Gustavo
UNSPECIFIED
Manuneedhi Cholan, Pradeep
UNSPECIFIED
Kim, Mikael
UNSPECIFIED
Rahman, Md Hafizur
UNSPECIFIED
Labbate, Maurizio
UNSPECIFIED
Rice, Scott A.
UNSPECIFIED
Pernice, Mathieu
UNSPECIFIED
Oehlers, Stefan H.
UNSPECIFIED
McDougald, Diane
UNSPECIFIED
Last Modified: 07 Dec 2021 09:48
URI: https://eprints.centenary.org.au/id/eprint/1165

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